Interleukin 15 (IL-15), also referred to as IL15 or IL-15, is a cytokine that shares structural similarities with IL-2. Like IL-2, IL-15 signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (γ-C, CD132). It is primarily secreted by mononuclear phagocytes, among other cells, in response to viral infections.
IL-15 plays a crucial role in the immune system by promoting the proliferation of natural killer (NK) cells - key players in the body's defense against virally infected cells. It also regulates the activation and expansion of T and NK cells. IL-15 provides essential survival signals that help maintain memory T cells without antigens and is also involved in NK cell development.
In rodent lymphocytes, IL-15 prevents apoptosis by inducing BCL2L1/BCL-x(L), an inhibitor of cell death. Furthermore, IL-15 has demonstrated the ability to enhance the anti-tumor immunity of CD8+ T cells in preclinical studies. A Phase I clinical trial, conducted by the National Institutes of Health, is currently enrolling patients to assess the safety, dosing, and anti-tumor efficacy of IL-15 in individuals with metastatic melanoma and renal cell carcinoma (kidney cancer).
Features
- Designed in accordance with ISO 13485:2016 and ISO 9001:2015
- Developed and QC-tested in a factory that complies with GMP
- FDA DMF filed
- Animal-free materials
- Beta-lactam materials free
- Batch-to-batch consistency
- Extensive quality control procedures
- No animal-derived peptone or lactose was used in the production process
Source
E. Coli cells express GMP Human IL-15 Protein (GMP-L15H13). AA Asn 49-Ser 162 (Accession # P40933-1) is present in it.
Predicted N-terminus: Met
Molecular characterization
Image Credit: ACROBiosystems Inc.
There is no "tag" on this protein.
The protein's calculated MW is 12.8 kDa. Under reducing (R) conditions, the protein migrates as 13 kDa±3 kDa (SDS-PAGE).
Endotoxin
The LAL technique yields less than 5.0 EU per vial.
Host cell protein
ELISA-tested protein: less than 0.5 ng/µg.
Host cell DNA
Protein tested using the DNA Fluorescent Staining method: <0.02 ng/μg.
Sterility
The membrane filtration method outlined in CP<1101>, USP<71>, and Eur. Ph. 2.6.1 was used to conduct the sterility test.
Mycoplasma
Negative
In vitro virus assay
Negative
Purity
>95%, according to SDS-PAGE.
Formulation
Obtained by lyophilizing a 0.22 μm filtered solution in a 25 mM His solution at pH 6.2 with protective agents.
Shipping
This product is supplied and shipped with blue ice.
Storage
Store it at -20 °C or lower for long-term storage as soon as it is received.
Avoid repeating the freeze-thaw cycle.
This product is stable after storage at:
- -20 °C to -70 °C for 5 years in lyophilized state.
- -70 °C for 12 months under sterile conditions after reconstitution.
ACRO quality management system
- QMS（ISO, GMP）
- Quality Advantages
- Quality Control Process
QC and assay protocol
SDS-PAGE
Image Credit: ACROBiosystems Inc.
SDS-PAGE of GMP Human IL-15 Protein in reducing (R) conditions. Coomassie Blue staining was applied to the gel. More than 95% of the protein is pure.
Bioactivity-Bioactivity CELL BASE
Image Credit: ACROBiosystems Inc.
CTLL-2 cell proliferation is stimulated by GMP Human IL-15 Protein (Cat. No. GMP-L15H13). When compared to the human IL-15 WHO International Standard (NIBSC code: 95/554), the specific activity of GMP Human IL-15 is > 8.00ⅹ106 IU/mg (QC tested).
Application data
Image Credit: ACROBiosystems Inc.
CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) cultivated human PBMCs for 13 days. The culture included 50 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), 5 ng/mL GMP Human IL-7 Protein (ACROBiosystems, Cat. No. GMP-L07H24), and 1, 10, or 100 ng/mL GMP Human IL-15 Protein (ACROBiosystems, Cat. No. GMP-L15H13). The outcome demonstrates that Acro's GMP Human IL-15 Protein activity is constant between batches.
Image Credit: ACROBiosystems Inc.
For 14 days, human PBMCs were cultivated in X-VIVO-15 with 50 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14), 5 ng/mL GMP Human IL-7 Protein (ACROBiosystems, Cat. No. GMP-L07H24), and 1, 10, or 100 ng/mL GMP Human IL-15 Protein (ACROBiosystems, Cat. No. GMP-L15H13). The results reveal that Acro's GMP Human IL-15 Protein activity remains consistent across batches.
Bioactivity-Stability
Image Credit: ACROBiosystems Inc.
According to the cell-based assay, the GMP Human IL-15 Protein (Cat. No. GMP-L15H13) is stable at 37 °C for 48 hours.
Image Credit: ACROBiosystems Inc.
In a real-time stability experiment conducted at 4 °C for three months, the cell-based assay demonstrates that GMP Human IL-15 Protein (Cat. No. GMP-L15H13) is stable at that temperature.
Image Credit: ACROBiosystems Inc.
The GMP Human IL-15 Protein (Cat. No. GMP-L15H13) is stable after three freezing and thawing cycles, according to the cell-based assay.
Image Credit: ACROBiosystems Inc.
The cell-based assay demonstrates batch-to-batch consistency between Acro's GMP and PG IL-15.