The PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-HPY53) is a neutralizing antibody created through the site-specific conjugation of PE to the Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions using proprietary technology.
ACROBiosystems Inc., offers another version of the PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-PY54A2) that contains proclin, produced using the same manufacturing process, with the exception of proclin. This proclin-containing product exhibits identical performance and can be stored at temperatures between 2-8 °C for a minimum of 12 months following reconstitution.
ACROBiosystems Inc. provides a premium-grade PE-labeled monoclonal anti-FMC63 antibody, Mouse IgG1 (Y45) (FM3-PY54G0), manufactured using the same production process but adhering to a more stringent quality control system that includes a thorough array of tests, such as sterility and endotoxin assessments.
This product is specifically designed for applications in cell isolation and cell culture during the early stages of preclinical research.
Application
Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). This product is NOT compatible with the streptavidin detection system.
Clone
Y45
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Specificity
Identifies the antigen-recognition domain of FMC63-derived CARs.
Immunogen
Recombinant FMC63 scFv derived from HEK293 cells
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Isotype control
The Isotype control is sold separately. The product information can be found in the Cat. No. DNP-PM1.
Recommended dilution
1:50
Formulation
Lyophilized from a 0.22 μm filtered solution in PBS, 0.5 % BSA, pH 7.4 with trehalose as protectant.
Reconstitution
Refer to the Certificate of Analysis (CoA) for detailed instructions on reconstitution.
ACROBiosystems Inc. recommends following the CoA protocol for optimal performance.
Storage
For extended storage, the product must be kept lyophilized at -20 °C or below.
Protect from light and refrain from subjecting it to repeated freeze-thaw cycles.
This product is stable after storage at:
- -70 °C for 12 months under sterile conditions after reconstitution.
- -20 °C to -70 °C for 24 months in lyophilized state.
ACRO Quality Management System
- QMS (ISO, GMP)
- Quality Advantages
- Quality Control Process
Performance data
Bioactivity-FACS
5e5 of the Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) and PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control (Cat. No. DNP-PM1) respectively, PE signal was used to evaluate the binding activity (QC tested). Image Credit: ACROBiosystems
Non-specificity of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) binding to CD3+ cells present in human PBMC. Human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-labeled monoclonal anti-FMC63 antibody, Mouse IgG1 (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed, and then analyzed with FACS. Both FITC and PE positive signals were used to evaluate the non-specific binding activity to human CD3+ cells (QC tested). Image Credit: ACROBiosystems